DL-endopeptidases function as both cell wall hydrolases and poly-γ-glutamic acid hydrolases

Microbial production of poly-γ-glutamic acid.

Poly-γ-glutamic acid (γ-PGA) is a natural, biodegradable and water-soluble biopolymer of glutamic acid. This review is focused on nonrecombinant microbial production of γ-PGA via fermentation processes. In view of its commercial importance, the emphasis is on L-glutamic acid independent producers (i.e. microorganisms that do not require feeding with the relatively expensive amino acid L-glutami...

CELL WALL HYDROLASES IN THE SEEDS OF Euphorbia heterophylla

1Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, CEP 14049-900, Ribeirão Preto, SP, Brasil; 2 Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, CP 4005, CEP 01061-970, São Paulo, SP, Brasil; 3 Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, CEP 1404090...

Acid hydrolases of trypanosomatid flagellates.

Homogenates from 5 species of Trypanosomatids were screened for the presence of a series of acid hydrolases. The insect flagellae, Crithidia sp., contains 5 enzymes reminiscent of plant parasitism, which were absent from or of very low specific activity in parasites of the genera, Trypanosoma and Leishmania. The latter mammalian parasites, on the other hand, exhibited higher acid proteinase and...

Molecular modeling of family GH16 glycoside hydrolases: potential roles for xyloglucan transglucosylases/hydrolases in cell wall modification in the poaceae.

Family GH16 glycoside hydrolases can be assigned to five subgroups according to their substrate specificities, including xyloglucan transglucosylases/hydrolases (XTHs), (1,3)-beta-galactanases, (1,4)-beta-galactanases/kappa-carrageenases, "nonspecific" (1,3/1,3;1,4)-beta-D-glucan endohydrolases, and (1,3;1,4)-beta-D-glucan endohydrolases. A structured family GH16 glycoside hydrolase database ha...

Molecular Modelling of Family GH16 Glycoside Hydrolases: Potential Roles for Xyloglucan Endotransglucosylases/Hydrolases in Cell Wall Modification in the Poaceae